UV-Vis – Ultraviolet-Visible Spectroscopy
Dynalene provides cost effective UV-Vis analysis of your product. We can assay your materials or develop methods to fit your needs. With our years of experience providing material assays and method development we have the ability to analyze a broad range of materials.
- Dyes and pigments
- Personal care products
- Metallic ions
UV-Vis is an analytical technique that measures the absorbance of visible or ultraviolet light at a single wavelength or over a spectral range. By measuring the absorbance of a compound in solution, and calculating the molar extinction coefficient of the compound from a standard, the concentration of that material can be quantified. For example, Sun Protection Factor (SPF) can be determined by assaying the known active ingredient or calculation of the overall UV protection a product provides. In some identification tests, a compound can be recognized based on the wavelength that absorbs the most light.
How UV-Vis works
A Deuterium-Tungsten lamp provides light that is passed through a diffraction grating allowing for single wavelengths to pass through the sample to a photodiode array detector. The absorbance at a specific wavelength or a range of wavelengths is measured. The light is passed simultaneously through the sample and a reference solvent and the ratio of the two beams is displayed as the measurement. The absorbance of a solution is proportional to the concentration of the absorbing species allowing for quantification of the analyte of interest.
Dynalene utilizes a Shimadzu UV-1900 Spectrophotometer to perform UV-Vis analysis on materials including consumer care products, pigments, coatings, organic conjugates (proteins, DNA, RNA) and metallic ions. This technique is a cost effective way of performing assays and characterizing your product.
5250 West Coplay Road
Whitehall, PA 18052
1.877.244-5525 | 610.262.9686