High Performance Liquid Chromatography (HPLC) is an analytical technique used to separate, identify and quantify compounds in a wide array of samples from a broad spectrum of the industries. It is the most versatile of all analytical techniques. The primary use for HPLC is the assay of active ingredients in your product. This provides assurance that the product meets labeled specifications. It also becomes important in monitoring the efficacy of product over time. Breakdown of the active ingredient is the primary concern of industry. Whether by natural aging, or the effects of storage conditions, understanding the stability of your product is of vital importance.
How it works
High Performance Liquid Chromatography is an analytical technique for separating compounds in a sample matrix. Separation is achieved by dissolving the sample in a suitable solvent and injecting it into a stream of solvent called the mobile phase. The mobile phase (and injected sample) is passed through a stationary phase (HPLC column). The compounds exit the column at different times based on the affinity the compounds have for the mobile phase and the stationary phase. Upon exiting the HPLC column they are detected and a signal is obtained electronically. There are multiple modes of separation including reverse phase, normal phase, ion exchange, and size exclusion.
Response at the detector of the HPLC is proportional to the amount of analyte injected. Analysis of samples in combination with standards with known analyte concentrations, allows for the concentration of the compound of interest to be determined.
Dynalene employs an Agilent 1200 Series HPLC equipped with a Diode Array Detector (DAD) to analyze non-volatile and/or polar compounds. With our expertise in HPLC Method Development we can analyze a wide range of sample types including pharmaceuticals, organic acids, industrial chemicals, cosmetics, dietary supplements, food and beverages or develop a HPLC method for your unique analysis needs.